Chargaff
To determine whether DNA is
responsible for differences
between species
Used paper chromatography to
isolate DNA, and then analyzed
the nucleotides via
spectrophotometry
Ratios of A:T and G:C in DNA are
always both 1:1
Different species have different
ratios of A and T to G and C (jan 1, 1944 – jan 1, 1952)