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May 31, 2020
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Experimental Time Line
The time line is planned experiment to regenerate data lost due to computer crash
Created by
aron
⟶ Updated 13 Dec 2017 ⟶
List of edits
Timelines by
aron
:
13 Dec 2017
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147
result submission timeline
Timeline of results I am planning to send as I regenerate da...
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Events
Comittee Meeting
Dissection Day, neurons harvested and plated
Neurons maturing and begin somatic and AIS tether force mesurments
Send data collected so far, membrane mechanical properties of somatic and AIS membrane
Order Rat
Some event happened
prepare dissection tools and autoclave
send completed data set of AIS and somatic membrane mechanics
Rat Dissection
new set of neuron matured, beging somatic membrane mechanics measurments with application of latrinculin, MBCD, nocodazole
Send AIS and somatic tether data
Order E-13 Rat
Dissection and neuron harvested
Cells matured, Begin tether imaging, depolarize cells with increase potassium in extracellular solution and measure tether force tether force
Final Comittee meeting
Send total data to Dr. Brownell
order Rat
dissection and neuron harvested
order rat
Dissection
Order Rat
dissection
send tether images
collect tether flourescence images
depolarize membrane using increased potassium, measure tether force
send AIS tether force
Review time line and progress and updated timeline such that defence come up after next committee meeting
more electrically evoked tether force measurment
more electrically evoked tether force measurment with application of MBCD, slycilate
more electrically evoked tether force measurment with application of MBCD and slycilate
