Gene editing
Category: Other
Updated: 5 Dec 2018
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Created byMaya Rowley
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DNA of human embryos edited for the first time.
Location: Sun Yat-sen University, China
Method: CRISPR-Cas9
Journal: Protein & Cell
Type of embryo: non-viable human
Disease treated: Beta thalassemia
Results: 11 of the 86 embryos injected with CRISPR-Cas9 had the faulty gene replacedPermission to edit the DNA of human embryos was granted
for the first time in the U.K. by the HFEA.
Location: Francis Crick Institute, U.K.
Method: CRISPR-Cas9
Journal: Nature
Outline: the gene Oct4, known to be highly expressed in
embryonic stem cells, was inactivated in order to
research early embryo development.DNA of human embryos edited for the second time
Location: The Third Affiliated Hospital of Guangzhou Medical University, China
Method: CRISPR-Cas9
Journal: Journal of Assisted Reproduction and Genetics
Type of embryo: non-viable human
Outline: a gene which confers resistance to HIV was inserted into embryos
Results: No off-target effects found (of the 28 sites examined).
46 embryos were injected with the CRISPR system. 26 embryos survived to the 8-cell stage,
and only 4 successfully had the gene inserted.DNA of viable human embryos edited for the first time
Location: U.S.
Method: CRISPR-Cas9
Journal: Nature
Type of embryo: Viable human
Disease treated: Hypertrophic cardiomyopathy
Results: No off-target effects found. One embryo had all of its cells
successfully modified, two were mosaics, and the other 3 embryos
were found not edited at all.First time a base editor is used to edit DNA of embryos
Location: Sun Yat-Sen University, China
Method: Base editor
Journal: Protein & Cell
Type of embryo:
Disease treated: beta thalassemia
Results: Efficiency was 7-25.9%. Most of the edited embryos were mosaics,
with percentage of repaired cells being 20-75%. No off-target effects were found,
but only the “top 10 potential off-target sites” were analysed, so there could have
been unintended edits in the unexamined parts of the genome.CRISPR-Cas9 used for the first time in eukaryotic cells
Location: Broad Institute, U.S.
Journal: Science
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