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Robert Hooke discovered "cells" in corkJansen credited with 1st compound microscopeLeeuwenhoek discovered protozoa; he saw bacteria some 9 years laterBrown described the cell nucleus in the cells of a n orchardSchleiden and Schwann proposed cell theoryAlbrecht Von Roelliker he realized that sperm cells and egg cells are also cellsN. Pringsheim observed how a sperm cells penitrated an egg cellRudolf Virchow; he is a physician, pathologist, and anthropogist. He expounds his famous conclusion: omnis cellula e cellula (the cells develope only from existing cells)Kolliker discribed mitrocondriaFlemming discribed chromosome behavior during mitosis.Germ cells are haploid chromosome theory of heredityGolgi discribed golgi apparatusBehavior is used differential centrifgation to seperate the nucleus from cytoplasmGey and coworkers established a continuous human cell line.Eagle systematically defined the nutritional needs of animal cells in culture.Meselson, Stahl and Vinograd developed density gradient centrifugation in cesium chloride solutions for separating nucleic acids.Ham introduced a defined serum-free medium. Cambridge Instruments produced the first commercial scanning electron microscope.Sato and colleagues publish papers showing that different cell lines require different mixtures of hormones and growth factors in serum-free media.Transgenic mice and fruit flies are produced. Mouse embryonic stem cell line established.Tsien identifies mutant of GFP with enhanced spectral propertiesMice are cloned from somatic cells.Hamilton and Baulcombe discover siRNA as part of post-transcriptional gene silencing (PTGS) in plants
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