Experimental Time Line
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Wurde aktualisiert 13 Dez 2017
The time line is planned experiment to regenerate data lost due to computer crash
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Created byaron
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Ereignisse
Comittee MeetingDissection Day, neurons harvested and platedNeurons maturing and begin somatic and AIS tether force mesurmentsSend data collected so far, membrane mechanical properties of somatic and AIS membraneOrder RatSome
event
happenedprepare dissection tools and autoclavesend completed data set of AIS and somatic membrane mechanicsRat Dissectionnew set of neuron matured, beging somatic membrane mechanics measurments with application of latrinculin, MBCD, nocodazoleSend AIS and somatic tether dataOrder E-13 RatDissection and neuron harvestedCells matured, Begin tether imaging, depolarize cells with increase potassium in extracellular solution and measure tether force tether forceFinal Comittee meetingSend total data to Dr. Brownellorder Ratdissection and neuron harvestedorder ratDissectionOrder Ratdissectionsend tether imagescollect tether flourescence imagesdepolarize membrane using increased potassium, measure tether forcesend AIS tether forceReview time line and progress and updated timeline such that defence come up after next committee meetingmore electrically evoked tether force measurmentmore electrically evoked tether force measurment with application of MBCD, slycilatemore electrically evoked tether force measurment with application of MBCD and slycilate
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