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August 1, 2025
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Cancer 2019 semester 1
Category:
Andere
Wurde aktualisiert:
13 Jan 2020
0
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237
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Created by
Cassidy Williams
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Ereignisse
Thawed 4T1 cells (40% confluency)
Micoplasma contamination
Thawed 4T1 cells
Plated 4T1 cells
CRISPR/Cas9 transfection
PCR machine programmed and ran for lysis on the transfected cells
DNA extraction on 4T1 cells
PCR to amplify lysis product
Gel electrophoresis with p53 primers 1 and from extracted DNA
RNA extraction from lysis product
Gel electrophoresis ran on extracted RNA
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